Contribution of sulfur-containing precursors to release of hydrogen sulfide in sludge composting.

Hydrogen sulfide (H2S) production during composting can impact the environment and human health. Especially during the thermophilic phase, H2S is discharged in large quantities. However, in sludge composting, the contributions of different sulfur-containing precursors to H2S fluxes, key functional microorganisms, and key environmental parameters for reducing H2S flux remain unclear. Analysis of cysteine (Cys), methionine (Met), and sulfate (SO42-) concentrations, multiple stepwise regression analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analysis of metagenomes showed that Cys was the main contributor to the production of H2S and that Met was among the main sources during the first three days of composting, while the SO42- contribution to H2S was negligible. Fifteen functional genera involved in the conversion of precursors to H2S were identified by co-occurrence network analysis. Only Bacillus showed high temperature resistance (>50 °C) and the ability to reduce H2S. Redundancy analysis showed that total carbon (64.0 %) and pH (23.3 %) had significant effects on functional bacteria. H2S had a quadratic relationship with sulfur-containing precursors. All microbial network sulfur-containing precursors metabolism modules showed a highly significant relationship with Cys.

Coupling network of hydrogen sulfide precursors and bacteria in kitchen waste composting.

This study was focused on the changes of hydrogen sulfide (H2S), its precursors, and microorganisms associated with its transformation during the composting process of kitchen waste. The results showed that the content of cysteine (Cys) and methionine (Met) decreased by 32.3 % and 57.5 % respectively, while the content of sulfate (SO42-) changed little during composting. The main release period of H2S was during the high-temperature period of composting, Cys was its main precursor. Based on network analysis, a total of 15 core genera associated with the conversion of H2S precursors were identified, and the transformation of the H2S precursor was mainly influenced by Filomicrobium. Temperature, pH, and TN levels had a positive effect on Filomicrobium. It could find a balance point by controlling these three factors to reduce the production of H2S.

Microbiota Community Structure and Interaction Networks within Dermacentor silvarum, Ixodes persulcatus, and Haemaphysalis concinna.

Ticks carry and transmit a variety of pathogens, which are very harmful to humans and animals. To characterize the microbial interactions in ticks, we analysed the microbiota of the hard ticks, Dermacentor silvarum, Ixodes persulcatus, and Haemaphysalis concinna, using 16S rRNA, showing that microbial interactions are underappreciated in terms of shaping arthropod microbiomes. The results show that the bacterial richness and microbiota structures of these three tick species had significant differences. Interestingly, the bacterial richness (Chao1 index) of all ticks decreased significantly after they became engorged. All the operational taxonomic units (OTUs) were assigned to 26 phyla, 67 classes, 159 orders, 279 families, and 627 genera. Microbial interactions in D. silvarum demonstrated more connections than in I. persulcatus and H. concinna. Bacteria with a high abundance were not important families in microbial interactions. Positive interactions of Bacteroidaceae and F_Solibacteraceae Subgroup 3 with other bacterial families were detected in all nine groups of ticks. This study provides an overview of the microbiota structure and interactions of three tick species and improves our understanding of the role of the microbiota in tick physiology and vector capacity, thus being conducive to providing basic data for the prevention of ticks and tick-borne diseases.